The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. 1. 72 MB. The advantage of using labeled probes is detection specificity. . For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The assay window spanned four orders of magnitude. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 1 second. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. book Page 7 Wednesday, July 7, 2010 2:09 PM. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. 26 1. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. 2. 04 to 3. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. 1. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. It measures the optical density (OD) of samples in a microplate or in a cuvette at aThe cells were then washed three times with PBS, and the fluorescent intensity of the cells was measured with a SpectraMax M5 microplate reader (Molecular Devices, USA) at an excitation wavelength of 488 nm and emission wavelength of 525 nm. RAW 264. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. 2 only). The cuvette must be in place when you read the plate. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. 5 12. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). The drawing shows a standard microplate of 15 mm height covered with a lid. 54 1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. used. 2. MOLECULAR DEVICES SpectraMax M5 also includes a wide selection of curve-fitting functions, allowing users to perform accurate and reliable quantification of biochemical reactions. 0 out of 5 $ 17,999 $ 16,995. SpectraMax M5e Multi-Mode Microplate Reader. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 2. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The following document is intended to demonstrate setup of this instrument. Fluorescence Microplate Reader Comparison Chart. 0 out of 5 $ 16,499. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Uncontrolled Copy for Training and Reference Purposes Only . Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 9000-0756. Five preferred modes for diverse bioresearch applications. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. com. edu) to be added as a trained user in iLab. 7°C (gradient heating design). The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Has anyone had problems with the built-in computer of the Spectramax ID3. Fill a clean plate with water. Cell viability was quantified by measuring the absorbance of the solution at 570 nm using a SpectraMax® M5 microplate reader (Molecular Devices). Read More. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. 6 0. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. Figure 4: Transcreener ADP. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Are you using the correct Microplate Adapters? For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Molecular Devices SpectraMax M5 ; Molecular Devices SpectraMax M2. Microplate Readers include the M3, M4, M5, and M5e models. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. Click SoftMax Pro Software User Guide. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. SpectraMax Lmicroplate luminometer. and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. ® reader to the computer. SpectraMax M Series multi-mode microplates are configurable readers with triple. Bookings are free, but you will need to use iLab to book. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Table of Contents. flexibility of monochromator-based systems. 4 Max StDev 1. AquaMax® 4000. Microplate Capacity. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Italic and boldface type are used for emphasis. 1 Version 7. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. 00 USD. Microplate Readers Biotek Synergy H1 Hybrid Microplate. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. 7. 2. 1 is GxP edition only; Protocols for the ABS1 Validation Plate. PMT sensitivity was set to AutoRange with a target calibration wavelength of 470 nm, and the plate was dark adapted for 1 minute prior to reading. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax M5 reader settings are shown. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. analysis, controls the SpectraMax Multi-Mode Microplate Readers. e. 00 USD. analysis, controls the SpectraMax Multi-Mode Microplate Readers. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. 00 USD. SpectraMax® ABS Plus. AdThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Background fluorescence has been subtracted. 5 87. #M5) SpectraMax® L Microplate Reader (cat. SpectraMax® M4. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. System Diagrams and Dimensions. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 2. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. book Page 7 Wednesday, July 7, 2010 2:09 PM. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. 95. These lab workhorses are used in drug discovery, research, bioassay validation, and. 3. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. . Sale price $18,499. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. Wide range of applications. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. Molecular Devices Spectramax M5 Microplate Reader. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Published Dec 16, 2020 | Updated Dec 16, 2020. for ValitaTiter Plus standards read on Molecular Devices readers. Shop now. This application note describes how to. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. The cells were then treated to induce apoptosis. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. For example,it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Ferguson, Ph. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. 67. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. Spectramax M5 microplate reader: Molecular Devices: N/A:. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. This assay is based on the disappearance of hydrogen peroxide (H 2 O 2) at 240 nm in a medium containing 20 mM H 2 O 2, 0. No filter change with dual monochromator optics. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideFluorescence Microplate Reader Comparison Chart. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Shimadzu 2450 UV–Vis spectrophotometer and SpectraMax M5 microplate reader were used to perform the optical characterization and measurements. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Ltd. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Self Service Available. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. M3-M5e_manual. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. Italic and boldface type are used for emphasis. 43 Max %CV 1. 00. SpectraMax® Aquamax. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. spr. The samples were boiled for 5 min with 1 ×. The cuvette must be in place when you read the plate. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Table of Contents. 1step assay with SpectraMax microplate readers. The following document is. Data are normalized to vehicle-treated. M3-M5e_manual. Molecular Devices. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. SpectraMax® M5e. With optimized reagents, option to operate the. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. Insert the filled microplate into the drawer, matching well A1 with position A1 in the drawer. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 2. SpectraMax® Microplate Readers and SoftMax® Pro Software. 314 low lamp level warning. Ltd. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Amino acids assay. Pick the best speed, sensitivity, and price for your needs. • Tel. 00 USD. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Molecular Devices Spectramax M5 Microplate Reader. However, when a specific probe is not available, or it causes off-target effects on the. In . The system. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Molecular Devices SpectraMax® series microplate readers. Molecular Devices SpectraMax™ Plus 384 Microplate Reader (s) with One-Year Warranty $9,000. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. AquaMax® 2000. Fluorescent signals were measured using the SpectraMax/M5 fluorescence spectrophotometer plate reader (Molecular Devices Co. 6: bioNova científica, s. Also refer to this Multimode Reader Comparison Chart . SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Fifteen different automated tests are provided. . S. Go to Product Details. ATG provides repair, maintenance, and contract services for laboratory research and bioprocess instruments. This is equivalent to having 511 built-in filters,The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. This application note discusses the principles on which PathCheck iswells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Physical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. Molecular Devices. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. HTRF-compatible SpectraMax Multi-Mode Readers. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Five preferred detection modes. When the user applies a set temperature of 37°C, the top is heated to 37. 7°C and the bottom to 36. 59 6. spectramax m5 multi. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. e. 2. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The dual monochromator optics allow the widest range of applications to be utilized for bior. These robust readers have been placed in labs from Antarctica to. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 5. The SpectraMax M5e Multi. 305 reference level saturation. view more . The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. FlexStation™ II. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3. Learn how you can utilize microplate readers to drive landmark discoveries. Microplate Readers Include the M3, M4, M5, and M5. Fluorescence Microplate Readers. The Spectr. Compatible automation solutions for SpectraMax Readers. SpectraMax Glo Steady-Luc Reporter Assay Kit. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The K d was calculated with GraphPad Prism software (GraphPad Software Inc. Here the delta mP was 336, and Z’ factor was 0. KEY FEATURES • Five. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. A serial dilution of IgG standards was performed, using XP Media/L-glutamine as the diluent, to concentrations from 2. B: Selection of Excitation Wavelength. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The spectrophotometric. Sensitive fluorescent quantitation of DNA with the Quant-iT. The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. edu) to be added as a trained user in iLab. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. We would like to show you a description here but the site won’t allow us. Microplate Readers are modular and . This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. Bioz Stars score: 86/100, based on 1 PubMed citations. Recertification is recommended every two years. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Maximum signal with lowest background. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. 2. When the user applies a set temperature of 37°C, the top is heated to 37. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Anne T. 4. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Table of Contents. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. The optical density (OD) was measured at 450 nm with a SpectraMax M5 microplate reader (Molecular Devices). Add to Cart. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 6 for further experiment, so I'm confused which i would choose for OD value determination. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. (Shanghai, China). SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The dual monochromator optics allow the. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. 49 2. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. SpectraMax M5 microplate reader (Molecular Devices, California, USA) with excitation at 450 nm and emission at 570 nm. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. SpectraMax® ABS Plus. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. FlexStation™ III. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. We would like to show you a description here but the site won’t allow us. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Our absorbance plate readers feature our PathCheck Sensor technology and. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 7°C (gradient heating design). The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Temperature control range from Ambient +4º to 50ºC. 72 MB. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Choose cables that meet the requirement described in this appendix. Retrieving Each. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. Microplate Readers. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-,. The bound bio-peptidomimetics were recognized by Eu 3+-SA (diluted using 1% BSA in TBS), and the fluorescence intensities were measured using a SpectraMax M5 microplate reader after adding 200 μL signal enhancement solution, which the parameters were set as the followed: excitation and emission wavelengths of 340 and 615 nm,. The concentration of antibodies was determined by using a spectrophotometer from Puxi General Instrument Co. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 1. SpectraMax® iD5. When a. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 73. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. After 16 hr of treatment, cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. 6-384. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Key applications: ELISA, nucleic acid, endotoxin testing. 3 1. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. The integration time suggested is 5-10 sec. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Or, with the reader specifications noted from the Instrument Description section of your reader's. Thank you!. The chamber contains springs that position the cuvette in the proper alignment for a read. • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP.